Detection of tube agglutination 37°C-only antibodies by solid-phase red cell adherence
نویسندگان
چکیده
منابع مشابه
Comparison of test tube, gel column agglutination and solid phase red cell adherence methods with and without drug added for detection of drug-dependent antibodies
BACKGROUND: Testing for drug-dependent antibodies (DDA) is traditionally performed in test tubes either with drug-treated RBCs or in the presence of drug. Gel microcolumn and solid phase red cell adherence (SPRCA) methods were evaluated for detection of DDA with and without drug. STUDY DESIGN AND METHODS: Forty-nine frozen DDA samples tested by tube methods were selected. Samples were tested wi...
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Platelet refractoriness is caused by HLA antibodies and platelet-specific antibodies. Current methods used to detect antiplatelet antibodies have limitations. Solid phase red cell adherence (SPRCA) lacks sensitivity and requires a second assay using chloroquine-treated intact platelets to specify the response due to anti-HLA. We modified SPRCA by using 2 types of antihuman platelet antibodies w...
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1. The agglutination titer for chicken red cells of freshly prepared or carefully stored suspensions of PR8 influenza virus, that is to say virus of maximum pathogenicity, was found to be proportional to the mouse lethal titer of the same preparations. 2. The agglutination titer of infected allantoic fluid procured in a standard way is relatively constant, regardless of the influenza strain use...
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A procedure for antibody detection and identification that utilizes affinity microcolumns to isolate IgG antibodies in a gel matrix containing Protein G and Protein A was commercially available in recent years. We evaluated this method (ReACT, Red Cell Affinity Column Technology, Immucor Co., Norcross, GA) as an alternative to standard tube agglutination testing, in an effort to minimize subjec...
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Hepatitis B virus is an important worldwide public health problem especially in areas where the prevalence are high, such as China, Southeast Asia, tropical Africa. In Thailand, the carrier rate is about 5-10% of the total population (Kamolrata nakul et ai, 1985; Poovorawan et ai, 1989). The detection of hepatitis B surface antigen (HBsAg) is one of the indicators of the hepatitis B infectious...
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ژورنال
عنوان ژورنال: Immunohematology
سال: 2020
ISSN: 0894-203X,1930-3955
DOI: 10.21307/immunohematology-2019-742